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primary antibodies against pstat1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc primary antibodies against pstat1
    Primary Antibodies Against Pstat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+pstat1/pm41051359-337-1-13
    Average 86 stars, based on 1 article reviews
    primary antibodies against pstat1 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Immunofluorescence:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Staining:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Marker:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Control:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Flow Cytometry:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Isolation:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Disruption:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    RNA Sequencing:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Expressing:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Western Blot:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Immunostaining:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Microscopy:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    BIA-KA:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Activity Assay:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).

    Quantitative Proteomics:

    Article Title: Fludarabine attenuates inflammation and dysregulated autophagy in alveolar macrophages via inhibition of STAT1/IRF1 pathway
    Article Snippet: For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.For blocking, membranes were incubated with 5% skim milk and incubated with primary antibodies for overnight at 4 °C.. Primary antibodies against pSTAT1 (Ser727), pSTAT1 (Tyr701), STAT1, IRF1, IRF7, LC3B, and β-actin were purchased from Cell Signaling Technology, (Danvers, MA, USA).. Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).Primary antibodies against p62 and inducible nitric oxide synthase (iNOS) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: A novel JAK/ROCK inhibitor, CPL409116, demonstrates potent efficacy in the mouse model of systemic lupus erythematosus.
    Article Snippet: Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.Nonspecific binding sites were blocked with 5% BSA, before an overnight incubation in protein-specific primary antibodies, followed by 1 h incubation in the appropriate HRP-conjugated secondary antibody.. Primary antibodies against pSTAT1 (Y701, #7649), pSTAT3 (Y705, #9138), STAT1 (#9172) were provided by Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA) STAT3 (#BD610190) was obtained from BD Biosciences (BD Biosciences, San Jose, California, USA) a-tubulin (#T9026) was supplied by Sigma Aldrich (Sigma Aldrich, Saint Louis, Missouri, USA).. HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).HRPconjugated secondary antibodies a-Rabbit (#7074) and a-Mouse (#7076) were obtained from Cell Signaling (Cell Signaling, Danvers, Massachusetts, USA).



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    Image Search Results


    Reduced STAT1 phosphorylation in the tumoral region of NSCLC. ( A – F ) Western blot analysis of pSTAT1 and actin expression in lung tissue samples from the tumoral, peri-tumoral and control region of patients with adenocarcinoma (ADC) ( N Control =5; N Peri-tumoral =5, N Tumoral =5). Bar charts show mean values of the protein expression levels of pSTAT1 α -Isoform ( C and D ) and pSTAT1 β -Isoform ( E and F ) relative to actin levels in ADC and in SCC, respectively. ( G ) Flow cytometry analysis of pSTAT1 + cells in the tumoral, the peri-tumoral and the control lung region of one representative patient with NSCLC. pSTAT1 staining was performed with the lung cell suspensions after 20 min of incubation at 37 °C in the presence or absence of IFN- γ . pSTAT1 + cells were gated on CD4 + , CD8 + or CD11b + cells, respectively. ( H ) Flow cytometry analysis of pSTAT1 + CD4 + and pSTAT1 + CD8 + T cells gated on lymphocytes as well as pSTAT1 + CD11b + cells gated on big cells in the control, the peri-tumoral and the tumoral lung region of one representative patient with NSCLC. Data are shown as mean values±s.e.m. using Student's t -test * P =0.05.

    Journal: British Journal of Cancer

    Article Title: Impaired T-bet-pSTAT1 α and perforin-mediated immune responses in the tumoral region of lung adenocarcinoma

    doi: 10.1038/bjc.2015.255

    Figure Lengend Snippet: Reduced STAT1 phosphorylation in the tumoral region of NSCLC. ( A – F ) Western blot analysis of pSTAT1 and actin expression in lung tissue samples from the tumoral, peri-tumoral and control region of patients with adenocarcinoma (ADC) ( N Control =5; N Peri-tumoral =5, N Tumoral =5). Bar charts show mean values of the protein expression levels of pSTAT1 α -Isoform ( C and D ) and pSTAT1 β -Isoform ( E and F ) relative to actin levels in ADC and in SCC, respectively. ( G ) Flow cytometry analysis of pSTAT1 + cells in the tumoral, the peri-tumoral and the control lung region of one representative patient with NSCLC. pSTAT1 staining was performed with the lung cell suspensions after 20 min of incubation at 37 °C in the presence or absence of IFN- γ . pSTAT1 + cells were gated on CD4 + , CD8 + or CD11b + cells, respectively. ( H ) Flow cytometry analysis of pSTAT1 + CD4 + and pSTAT1 + CD8 + T cells gated on lymphocytes as well as pSTAT1 + CD11b + cells gated on big cells in the control, the peri-tumoral and the tumoral lung region of one representative patient with NSCLC. Data are shown as mean values±s.e.m. using Student's t -test * P =0.05.

    Article Snippet: Polyacrylamide gel electrophoresis was performed at 80 V for 1.5 h. Proteins were then transferred to a nitrocellulose membrane (PAGEr Ex Gels, Lonza, Rockland, USA) at 200 mA for 45 min. After a washing step and blocking of the membrane for 1 h (5% milk powder, 0.1% Tween-20 in PBS) at room temperature, the primary antibody against pSTAT1 (Cell Signaling Technology, Danvers, MA< USA) was applied and incubated overnight at 4 °C.

    Techniques: Phospho-proteomics, Western Blot, Expressing, Control, Flow Cytometry, Staining, Incubation